Identification of Phytophthora spp. isolated from plants and soil samples on strawberry plantations in Poland     

Meszka  Beata  Michalecka  Monika 《植物病害和植物保护杂志》2016,123(1):29-36

Journal of Plant Diseases and Protection - Crown and leather rot of strawberry caused by Phytophthora spp. are major soil diseases of cultivated strawberry...  相似文献   

The vascular plant colonization on decaying Picea abies logs in Karkonosze mountain forest belts: the effects of forest community type,cryptogam cover,log decomposition and forest management     

Monika Staniaszek-Kik  Jan Żarnowiec  Damian Chmura 《European Journal of Forest Research》2016,135(6):1145-1157

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Monitoring adventitious presence of transgenes in ex situ okra (Abelmoschus esculentus) collections conserved in genebank: a case study     

Rakesh Kumar Bairwa  Monika Singh  Rajesh K. Bhoge  Chitra Devi  Gurinderjit Randhawa 《Genetic Resources and Crop Evolution》2016,63(2):175-184

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Bioremediation of the soil contaminated with petroleum oil products using sewage sludge     

Viktorija Jakubauskaite  Zilvinas Kryzevicius  Dalia Ambrazaitiene  Monika Vilkiene  Danute Karcauskiene 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(8):664-670

ABSTRACT

The remediation technologies of soils contaminated with petroleum products are developed in two main directions: the first one encompasses searching for new effective bioagents and the other one explores the ways to activate those microorganisms present in the soil that are capable of degrading oil. The objective of this research was to determine if it is possible to increase the effectiveness of biodegradation of petroleum products by using chemical additives. The soil was supplemented with additives: CuSO4, MnSO4, KMnO4, H2O2, 5% and 10% chemical industry plants sludge, 5% and 10% Stock Company ‘Klaipedos vanduo’ (SC‘KV’) municipal wastewater treatment plants sludge. The data suggest that all the additives statistically significantly stimulated the degradation of diesel fuel (F = 12.01; p = .001) and black oil (F = 9.93; p = .001) compared with the control. It was determined that diesel fuel was degraded the fastest in samples with KMnO4, where efficiency of degradation was 90%, and 88% efficency in samples with 10% chemical industry plants sludge. Black oil was degraded the best in samples, where KMnO4 was added: efficiency of degradation was up to 63%. In the samples with 10% of sewage sludge from chemical industry plant degradation efficiency was 62%.  相似文献   

Agrobacterium-mediated Cry1A(b) gene transfer in Punica granatum L. cv. Kandhari Kabuli using different In Vitro regeneration pathways     

Vipasha Verma  Kamlesh Kanwar  Mahak Tufchi  Monika Kashyap 《Journal of Crop Science and Biotechnology》2014,17(1):1-10

Three different regeneration systems, viz. regeneration through callus cultures using embryonic explant, direct regeneration using shoot bud/nodal segments as explant and regeneration through cell suspension culture using cotyledonary explant (for the induction of transgenic callus for suspension culture) were evaluated to see their effect on transfer of Cry1A(b) gene to Punica granatum L. cv. Kandhari Kabuli through Agrobacterium mediated transformation. Pre-conditioning and co-cultivation durations had a marked effect on transformation frequency of different explants. Out of different explants used (embryo, shoot bud, and cotyledon) for different regeneration systems cotyledonary explant showed highest putative transformation frequency (13.54%) inducing callus on selective medium for carrying out cell suspension culture to regenerate transgenic shoots. Despite of the highest transformation frequency obtained from the cotyledon explant, the plating efficiency of the transgenic cells generated through the transgenic callus (callus formed from the cotyledonary explant) during cell suspension culture was found to be very low (0.7%). Thus the plating efficiency has also played worth mentioning role in the regeneration of transformants following cell suspension culture. Among the three regeneration systems, regeneration through callus cultures using embryonic explant was found to be best for regeneration of transformants. The highest per cent regeneration of 23.33 was obtained from the putative transgenic embrogenic calli. Successful genetic transformation in the transformed plantlets was confirmed by PCR analysis. The transformation system thus developed is valuable and may be used to produce insect resistant trees.  相似文献   

Evaluation of low-molecular-weight heparin for the prevention of equine laminitis after colic surgery     

Geoffroy de la Rebière de Pouyade  DVM  MSc  ; Sigrid Grulke  DVM  PhD  DECVS  ; Johann Detilleux  DVM  MSc  PhD  DECVPH  ; Alexandra Salciccia  DVM  MSc  ; Denis R. Verwilghen  DVM  MSc  ; Isabelle Caudron  DVM  PhD  DECVS  ; Monika Gangl  MV  MSc  DECVS    Didier D.A. Serteyn  DVM  PhD  DECVA 《Journal of Veterinary Emergency and Critical Care》2009,19(1):113-119

Objectives – The aim of this study is to describe the prevalence of postoperative laminitis in colic cases and to determine if low-molecular-weight heparin (LMWH) is effective in preventing this complication.
Design – Retrospective clinical study.
Animals – Client-owned horses.
Interventions – SC administration of enoxaparin during the postoperative period.
Measurements and Main Results – Medical records of 360 horses undergoing surgery for colic and surviving at least 3 days were evaluated. Fifty-six horses admitted before 1995 did not receive LMWH (control group) and 304 admitted after 1995 received LMWH as a prophylaxis for laminitis (treatment group). Three grades of severity were defined for laminitis. Prevalence and severity of laminitis were compared between the 2 groups. Several parameters recorded on admission (sex, age, breed, site and nature of the disease, heart rate, PCV, gravity score, and shock score) and the administration of LMWH were tested as risk factors in the development of laminitis in a logistic regression procedure. Prevalence and grade of laminitis were significantly lower in the treatment group. Only the absence of LMWH was recognized as a significant risk factor in the logistic regression model.
Conclusions – The administration of LMWH appears to be effective in the prophylaxis of laminitis following colic surgery and may be useful in the postoperative management of these horses.  相似文献   

Wilderness Mass Casualty Incident (MCI): Rescue Chain After Avalanche at Everest Base Camp (EBC) In 2015     

Ken Zafren  Anne Brants  Katie Tabner  Andrew Nyberg  Matiram Pun  Buddha Basnyat  Monika Brodmann Maeder 《The Forestry Chronicle》2018,29(3):401-410

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Titanium Dioxide Nanoparticle Circulation in an Aquatic Ecosystem     

Monika Asztemborska  Małgorzata Jakubiak  Romuald Stęborowski  Ewelina Chajduk  Grażyna Bystrzejewska-Piotrowska 《Water, air, and soil pollution》2018,229(6):208

Nanotechnology is a dynamically developing field of scientific and industrial interest across the entire world, and the commercialization of nanoparticles (NPs) is rapidly expanding. Incorporation of nanotechnologies into a range of manufactured goods results in increasing concern regarding the subsequent release of engineered NPs into the environment. One of the biggest threats of using NPs is the transfer and magnification of these particles in the trophic chain. The aim of the studies was the evaluation of the distribution of TiO₂ NP contamination in the aquatic ecosystem under laboratory conditions. Bioaccumulation of TiO₂ NPs by plants (Elodea canadensis) and fish (Danio rerio) in the source of contamination was investigated. The studies were focused on the consequences of short-term water contamination with TiO₂ NPs and the secondary contamination of the components of the investigated model ecosystem (plants, sediments). It was found that in the fish and the plants exposed to NP contamination, the amount of Ti was higher than in the control, indicating an effective bioaccumulation of NPs or ions originating from NPs. It was clearly shown that the NPs present in the sediments are available to plants and fish. Additionally, the aquatic plants, an important trophic level in the food chain, can accumulate NPs and be a source of NPs for higher organisms. It was concluded that even an incidental contamination of water by NPs may result in long-term consequences induced by the release of NPs.  相似文献   

Concentration‐dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans          下载免费PDF全文

Monika M Kudelska  Lindy Holden‐Dye  Vincent O'Connor  Declan A Doyle 《Pest management science》2017,73(7):1345-1351

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Nonregenerative immune‐mediated anemia associated with a diffuse large B‐cell lymphoma in a captive jaguar (Panthera onca)          下载免费PDF全文

Monika A. Keresztes  Manfred Henrich  Penelope Baloi  Sascha Gerst  Jens‐Christian Rudnick  Judith Langenstein  Andreas Moritz  Natali Bauer 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(4):597-604

An 18‐year‐old male castrated jaguar (Panthera onca) was presented with anorexia and continuous bleeding from the oral cavity after a history of fighting with the partner animal. Clinical evaluation revealed ulcerating lesions on the gingiva and hard palate and a hematoma on the tongue. Computed tomography of the head and endoscopic examination of the esophagus and stomach were unremarkable. Hematology and clinical chemistry revealed severe nonregenerative anemia, mild thrombocytopenia, and moderate azotemia. Several PCRs for feline hemotropic mycoplasmas (Mycoplasma haemofelis, M heamominutium, M turicensis), Babesia felis, and Bartonella spp., as well as an FeLV antigen test were negative. The cytologic examination of a bone marrow aspirate was consistent with ineffective erythropoiesis, most likely due to immune‐mediated destruction of the erythroid precursor cells. Prednisolone therapy was initiated (1.25 mg/kg/day), and the CBC returned to normal 16 days after the initiation of the therapy. Anemia relapsed after 4 months and severe splenomegaly was noted. A repeat bone marrow aspirate revealed active erythropoiesis in the presence of erythroid precursor phagocytosis suggesting an immune‐mediated process. Splenic fine‐needle aspiration and tissue biopsies were taken, and all findings including histology and immunohistochemistry were consistent with a diffuse large B‐cell lymphoma (DLBCL). Five days later, the clinical condition deteriorated and the jaguar died. Histopathology following necropsy showed infiltration with neoplastic lymphoblasts in the spleen, liver, and abdominal lymph nodes. This case report describes a nonregenerative immune‐mediated anemia associated with a DLBCL in a jaguar.  相似文献